The seasonal activity and the effect of mechanical bending and wounding on the PtCOMT promoter in Betula pendula Roth.
Identifieur interne : 003A50 ( Main/Exploration ); précédent : 003A49; suivant : 003A51The seasonal activity and the effect of mechanical bending and wounding on the PtCOMT promoter in Betula pendula Roth.
Auteurs : Heidi Tiimonen [Finlande] ; Hely H Ggman ; Chung-Jui Tsai ; Vincent Chiang ; Tuija AronenSource :
- Plant cell reports [ 0721-7714 ] ; 2007.
Descripteurs français
- KwdFr :
- Betula (génétique), Betula (métabolisme), Contrainte mécanique (MeSH), Racines de plante (génétique), Racines de plante (métabolisme), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes végétaux (MeSH), Saisons (MeSH), Tiges de plante (anatomie et histologie), Tiges de plante (génétique), Tiges de plante (métabolisme), Végétaux génétiquement modifiés (MeSH).
- MESH :
- anatomie et histologie : Tiges de plante.
- génétique : Betula, Racines de plante, Régions promotrices (génétique), Tiges de plante.
- métabolisme : Betula, Racines de plante, Tiges de plante.
- Contrainte mécanique, Régulation de l'expression des gènes végétaux, Saisons, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Betula (genetics), Betula (metabolism), Gene Expression Regulation, Plant (MeSH), Plant Roots (genetics), Plant Roots (metabolism), Plant Stems (anatomy & histology), Plant Stems (genetics), Plant Stems (metabolism), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (genetics), Seasons (MeSH), Stress, Mechanical (MeSH).
- MESH :
- anatomy & histology : Plant Stems.
- genetics : Betula, Plant Roots, Plant Stems, Promoter Regions, Genetic.
- metabolism : Betula, Plant Roots, Plant Stems.
- Gene Expression Regulation, Plant, Plants, Genetically Modified, Seasons, Stress, Mechanical.
Abstract
In this study, 900-bp (signed as p including nucleotides -1 to -886) and partly deleted (signed as dp including nucleotides -1 to -414) COMT (caffeate/5-hydroxyferulate O-methyltransferase) promoters from Populus tremuloides Michx. were fused to the GUS reporter gene, and the tissue-specific expression patterns of the promoters were determined in Betula pendula Roth along the growing season, and as a response to mechanical bending and wounding. The main activity of the PtCOMTp- and PtCOMTdp-promoters, determined by the histochemical GUS assay, was found in the developing xylem of stems during the 8th-13th week and in the developing xylem of roots in the 13th week of the growing season. The GUS expression patterns did not differ among the xylem cell types. The PtCOMT promoter-induced GUS expression observed in phloem fibres suggests a need for PtCOMT expression and thus syringyl (S) lignin synthesis in fibre lignification. However, the PtCOMTdp-promoter induced GUS expression in stem trichomes, which may contribute to the biosynthesis of phenylpropanoid pathway-derived compounds other than lignin. Finally, a strong GUS expression was induced by the PtCOMT promoters in response to mechanical stem bending but not to wounding. The lack of major differences between the PtCOMTp- and PtCOMTdp-promoters suggests that the deleted promoter sequence (including nucleotides -415 to -886) did not contain a significant regulatory element contributing to the GUS expression in young B. pendula trees.
DOI: 10.1007/s00299-007-0331-x
PubMed: 17431633
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Tsai">Chung-Jui Tsai</name></author><author><name sortKey="Chiang, Vincent" sort="Chiang, Vincent" uniqKey="Chiang V" first="Vincent" last="Chiang">Vincent Chiang</name></author><author><name sortKey="Aronen, Tuija" sort="Aronen, Tuija" uniqKey="Aronen T" first="Tuija" last="Aronen">Tuija Aronen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17431633</idno><idno type="pmid">17431633</idno><idno type="doi">10.1007/s00299-007-0331-x</idno><idno type="wicri:Area/Main/Corpus">003B81</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003B81</idno><idno type="wicri:Area/Main/Curation">003B81</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003B81</idno><idno type="wicri:Area/Main/Exploration">003B81</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The seasonal activity and the effect of mechanical bending and wounding on the PtCOMT promoter in Betula pendula Roth.</title><author><name sortKey="Tiimonen, Heidi" sort="Tiimonen, Heidi" uniqKey="Tiimonen H" first="Heidi" last="Tiimonen">Heidi Tiimonen</name><affiliation wicri:level="1"><nlm:affiliation>Finnish Forest Research Institute, Punkaharju Research Unit, Finlandiantie 18, 58450 Punkaharju, Finland. heidi.tiimonen@metla.fi</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Finnish Forest Research Institute, Punkaharju Research Unit, Finlandiantie 18, 58450 Punkaharju</wicri:regionArea><wicri:noRegion>58450 Punkaharju</wicri:noRegion></affiliation></author><author><name sortKey="H Ggman, Hely" sort="H Ggman, Hely" uniqKey="H Ggman H" first="Hely" last="H Ggman">Hely H Ggman</name></author><author><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name></author><author><name sortKey="Chiang, Vincent" sort="Chiang, Vincent" uniqKey="Chiang V" first="Vincent" last="Chiang">Vincent Chiang</name></author><author><name sortKey="Aronen, Tuija" sort="Aronen, Tuija" uniqKey="Aronen T" first="Tuija" last="Aronen">Tuija Aronen</name></author></analytic><series><title level="j">Plant cell reports</title><idno type="ISSN">0721-7714</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Betula (genetics)</term><term>Betula (metabolism)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Plant Roots (genetics)</term><term>Plant Roots (metabolism)</term><term>Plant Stems (anatomy & histology)</term><term>Plant Stems (genetics)</term><term>Plant Stems (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (genetics)</term><term>Seasons (MeSH)</term><term>Stress, Mechanical (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Betula (génétique)</term><term>Betula (métabolisme)</term><term>Contrainte mécanique (MeSH)</term><term>Racines de plante (génétique)</term><term>Racines de plante (métabolisme)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Saisons (MeSH)</term><term>Tiges de plante (anatomie et histologie)</term><term>Tiges de plante (génétique)</term><term>Tiges de plante (métabolisme)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Plant Stems</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betula</term><term>Plant Roots</term><term>Plant Stems</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Betula</term><term>Racines de plante</term><term>Régions promotrices (génétique)</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Betula</term><term>Plant Roots</term><term>Plant Stems</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Betula</term><term>Racines de plante</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term><term>Seasons</term><term>Stress, Mechanical</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Contrainte mécanique</term><term>Régulation de l'expression des gènes végétaux</term><term>Saisons</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, 900-bp (signed as p including nucleotides -1 to -886) and partly deleted (signed as dp including nucleotides -1 to -414) COMT (caffeate/5-hydroxyferulate O-methyltransferase) promoters from Populus tremuloides Michx. were fused to the GUS reporter gene, and the tissue-specific expression patterns of the promoters were determined in Betula pendula Roth along the growing season, and as a response to mechanical bending and wounding. The main activity of the PtCOMTp- and PtCOMTdp-promoters, determined by the histochemical GUS assay, was found in the developing xylem of stems during the 8th-13th week and in the developing xylem of roots in the 13th week of the growing season. The GUS expression patterns did not differ among the xylem cell types. The PtCOMT promoter-induced GUS expression observed in phloem fibres suggests a need for PtCOMT expression and thus syringyl (S) lignin synthesis in fibre lignification. However, the PtCOMTdp-promoter induced GUS expression in stem trichomes, which may contribute to the biosynthesis of phenylpropanoid pathway-derived compounds other than lignin. Finally, a strong GUS expression was induced by the PtCOMT promoters in response to mechanical stem bending but not to wounding. The lack of major differences between the PtCOMTp- and PtCOMTdp-promoters suggests that the deleted promoter sequence (including nucleotides -415 to -886) did not contain a significant regulatory element contributing to the GUS expression in young B. pendula trees.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17431633</PMID><DateCompleted><Year>2007</Year><Month>10</Month><Day>26</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0721-7714</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>8</Issue><PubDate><Year>2007</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>The seasonal activity and the effect of mechanical bending and wounding on the PtCOMT promoter in Betula pendula Roth.</ArticleTitle><Pagination><MedlinePgn>1205-14</MedlinePgn></Pagination><Abstract><AbstractText>In this study, 900-bp (signed as p including nucleotides -1 to -886) and partly deleted (signed as dp including nucleotides -1 to -414) COMT (caffeate/5-hydroxyferulate O-methyltransferase) promoters from Populus tremuloides Michx. were fused to the GUS reporter gene, and the tissue-specific expression patterns of the promoters were determined in Betula pendula Roth along the growing season, and as a response to mechanical bending and wounding. The main activity of the PtCOMTp- and PtCOMTdp-promoters, determined by the histochemical GUS assay, was found in the developing xylem of stems during the 8th-13th week and in the developing xylem of roots in the 13th week of the growing season. The GUS expression patterns did not differ among the xylem cell types. The PtCOMT promoter-induced GUS expression observed in phloem fibres suggests a need for PtCOMT expression and thus syringyl (S) lignin synthesis in fibre lignification. However, the PtCOMTdp-promoter induced GUS expression in stem trichomes, which may contribute to the biosynthesis of phenylpropanoid pathway-derived compounds other than lignin. Finally, a strong GUS expression was induced by the PtCOMT promoters in response to mechanical stem bending but not to wounding. The lack of major differences between the PtCOMTp- and PtCOMTdp-promoters suggests that the deleted promoter sequence (including nucleotides -415 to -886) did not contain a significant regulatory element contributing to the GUS expression in young B. pendula trees.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tiimonen</LastName><ForeName>Heidi</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Finnish Forest Research Institute, Punkaharju Research Unit, Finlandiantie 18, 58450 Punkaharju, Finland. heidi.tiimonen@metla.fi</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Häggman</LastName><ForeName>Hely</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Tsai</LastName><ForeName>Chung-Jui</ForeName><Initials>CJ</Initials></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>Vincent</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Aronen</LastName><ForeName>Tuija</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>03</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D029662" MajorTopicYN="N">Betula</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012621" 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Tuija" sort="Aronen, Tuija" uniqKey="Aronen T" first="Tuija" last="Aronen">Tuija Aronen</name><name sortKey="Chiang, Vincent" sort="Chiang, Vincent" uniqKey="Chiang V" first="Vincent" last="Chiang">Vincent Chiang</name><name sortKey="H Ggman, Hely" sort="H Ggman, Hely" uniqKey="H Ggman H" first="Hely" last="H Ggman">Hely H Ggman</name><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name></noCountry><country name="Finlande"><noRegion><name sortKey="Tiimonen, Heidi" sort="Tiimonen, Heidi" uniqKey="Tiimonen H" first="Heidi" last="Tiimonen">Heidi Tiimonen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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